Phee401 载体

Author: rjkp

August undefined, 2024

Web载体骨架基本信息: 载体名称: pCambia: 载体出品公司:--载体抗性: 卡那霉素: 空载体大小: 6500 bp: 完整质粒大小:--载体修饰:--载体类型: 植物表达；植物二元向量: 筛选标记: 新霉 … http://www.biofeng.com/adjy/zhili/pHEE401E.html

pHEE401E载体图谱质粒图谱、序列、价格、抗性、测序引物、大 …

Web将转有pHEE401质粒的农杆菌GV3101在液体Luria-Bertani(LB)培养基(含抗生素利福平、卡那霉素和庆大霉素)中,于28 ℃恒温振荡培养36 h.离心收集培养好的农杆菌,采用菌体重悬 … WebDownload over 2,700 carefully annotated plasmid and sequence files including commonly used cloning vectors from all major suppliers. Open sequence files in SnapGene to view features, annotate, clone and use as a reference. Display enzyme sites, features, primers, ORFs, translations and more on plasmid maps or in detail on the sequence view. how is appreciation communicated

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http://www.biofeng.com/adjy/zhili/pKSE401.html WebMar 11, 2024 · We thank Dr. Qijun Chen for sharing the plasmids pHEE401 and pCBC-DT1T2, Dr. Donald P. Weeks for sharing the vector pCAMBIA-Cas9. This work was supported by the Shenzhen Science and Technology Innovation Committee (JCYJ20240818100038326), Natural Science Foundation of Guangdong Province (2024A030313966), National Natural … WebName. pHEE401. Type. plasmid. Tair Accession. Vector:6531224344. Description. CRISPR/Cas9 vector with maize codon-optimized Cas9 between egg cell-specific promoter (EC1.2) and rbcS-E9 terminator. Host Strain. high isletm collector\\u0027s edition

Phee401 载体

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Web将载体phee401 e-gmptl-1、phee401e-gmptl-2和phee401e-gmap1-1转化入农杆菌lba4404中，通过优化后的根癌农杆菌介导的子叶节转化体系对大豆基因组进行编辑，获得了t1代株系7株。 (4)用特异性引物扩增7株t1代株系靶位点附近碱基序列，发现共有6株大豆的基因发生了 … http://biovector.net/product/427522.html

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WebCurrent local time in USA – Illinois – Chicago. Get Chicago's weather and area codes, time zone and DST. Explore Chicago's sunrise and sunset, moonrise and moonset. WebpHEE401E. Plasmid. #71287. Purpose. Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 …

WebApr 14, 2024 · Carl D. Amore. Waukesha, WI - Died on April 8, 2024 at Waukesha Memorial Hospital at the age of 87. He was born in Chicago, IL on Aug. 30, 1935, the son of Charles …

WebDec 6, 2024 · 第一个是适应阶 CRISPR/Cas9 基因编辑载体pKSE401 的优化及其应用段，细菌和古细菌的外源小片段DNA间隔序列前体（protospacers）整合到CRISPR 系统中的 … how is apps stockWebMar 20, 2024 · 农杆菌介导的转化是植物转基因的常规方法，但在已经发表的8篇文章中，需要两个克隆步骤才能完成农杆菌双元载体的构建：第一步构建gRNA表达框；第二步 … how is apr calculated on a savings accountWebMar 2, 2024 · 1.5 pHEE401-mCherry-CKX表达载体的构建. 1.5.1CKX3sgRNA片段的扩增. 以pCBC 质粒（含gRNA）为模板，CKX3-F 和CKX3-R 为引物，进行PCR 扩增，反应程序：预变性94℃4 min，35 个循环（变性94℃30 s，退火56℃30 s，延伸72℃45 s），总延伸72℃2 min，4℃保存。 high isle wayshrine locationsWebpHEE401 (~100 ng/μl) 2 μl 10× T4 DNA Ligase Buffer (NEB) 1.5 μl 10× BSA 1.5 μl BsaI (NE B) 1 μl T4 DNA Ligase (HC, NEB) 1 μl ddH 2 O 6 μl Total volume 15 μl 5. Transform E.coli competent cells with 5 μl of reaction mixture, and select … high isle wayshrineWeb载体介绍 CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg … high isle upgradeWebOct 26, 2024 · sgRNA 是大名鼎鼎的基因编辑神器 Cas9 系统的“指挥中心”，可以引导 Cas9 对 DNA 进行定点编辑。. 经过无数大牛的积极探索，逐渐成熟的 Cas 9 基因编辑技术因其操作简单、编辑效率高等优势而席卷全球。. 因此，Cas9 基因编辑技术被称为诺贝尔奖级别的发明。. … how is aprc calculatedWebPlasmid pHSN401 from Dr. Qi-Jun Chen's lab contains the inserts 3×FLAG-NLS-zCas9-NLS and gRNA scaffold and is published in BMC Plant Biol. 2014 Nov 29;14(1):327. This plasmid is available through Addgene. high isle treasure map v eso