WebThe methods of callus induction and differentiation were studied by using Gerbera delavayi leaves as explants. The results showed that the green callus with diameter 0.5 cm, compact structure and hard texture could be induced from the aseptic leaves of Gerbera delavayi in the medium of WPM+ 0.6 mg·L −1 6-BA + 0.45 mg·L−1 NAA + 30 … WebAug 13, 2024 · Transgenic callus (TC) lines were selected on callus induction medium [CI medium: Murashige and Skoog ... (2024) Enhancement of recombinant protein production in transgenic Nicotiana benthamiana plant cell suspension cultures with co-cultivation of Agrobacterium containing silencing suppressors. Int J Mol Sci 19:1561. Article Google …
Direct delivery and fast-treated Agrobacterium co-culture …
WebJul 21, 2024 · Vitis vinifera plants are disease-susceptible while Vitis pseudoreticulata plants are disease-resistant; however, the molecular mechanism remains unclear. In this study, the single-stranded DNA- and RNA-binding protein gene Whirly (VvWhy1 and VpWhy1) were cloned from V. vinifera “Cabernet Sauvignon” and V. pseudoreticulata “HD1”. WebCallus induction rate and regeneration capacity of callus were greatly influenced by the genotype. Data analysis showed a callus induction rate of 88.5% and 58.3%, … family planning associates medical group ca
Callus Induction and in vitro Complete Plant Regeneration
WebSep 29, 2024 · Here, we describe a method for highly efficient targeted mutagenesis in Nicotiana benthamiana through the expression of Cas9 and single-guide (sg)RNA using a potato virus X ... surface-sterilized leaves at 7 dai were placed onto callus and shoot induction medium (Fig. 2A). Cefotaxime was added to the medium to kill Agrobacterium, ... WebMES Monohydrate (solid) 30.00 g. Agar - Plant Cell Culture (solid) 2000.0 ul. (2,4-Dichlorophenoxy)acetic Acid Sodium Salt, 1 mg/ml. Add after autoclaving other ingredients and cooling to ~60°C. 2000.0 ul. Kinetin, 0.1 mg/ml. Add after autoclaving other ingredients and cooling to ~60°C. WebNicotiana benthamiana is a commonly used model plant for studying plant–pathogen interactions because it is host to numerous plant pathogens and because many research tools are available for this species. ... 1 mg/L NAA, and 0.3 mg/L kinetin (1N0.3K) for plant regeneration. Callus formation occurred using protoplasts after 1 month of ... cool grips cell phone holder